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After a 3-h incubation at 37 °C, the media was changed and the following agents added at the indicated dose and duration: murine recombinant TNFα (R&D) (20 ng/mL), murine recombinant IFNγ (Peprotech) (50 ng/mL), IL1β(R&D) (20 ng/mL), LPS (1μg/mL), or DETA/NO (100–500 μM)

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Redox Biol (2013) 1(1):304–12